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1 x mops separation buffer  (Thermo Fisher)


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    Structured Review

    Thermo Fisher 1 x mops separation buffer
    1 X Mops Separation Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/1+x+mops+buffer/1+x+mops+separation+buffer/pm30208333-125-19-23
    Average 90 stars, based on 1 article reviews
    1 x mops separation buffer - by Bioz Stars, 2026-10
    90/100 stars

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    other:

    Article Title: Nance-Horan Syndrome-like 1 interacts with endophilin and Ena/VASP proteins to promote fast endophilin-mediated endocytosis
    Article Snippet: 5% β-mercaptoethanol was added to each sample and boiled at 70 °C for 10 min before running on 4-12% Bis-Tris gradient gels (Invitrogen) with 1 x MOPS buffer (Invitrogen).

    Article Title: S . pombe wtf drivers use dual transcriptional regulation and selective protein exclusion from spores to cause meiotic drive
    Article Snippet: We then ran the proteins on NuPAGE 4–12% Bis-Tris protein gels (Life Technologies) in 1 x MOPS buffer (Life Technologies) for 50 minutes at 200V and then transferred to PVDF membranes (Bio-RAD #1704156) using the Trans-Blot Turbo system.

    Article Title: S. pombe wtf drivers use dual transcriptional regulation and selective protein exclusion from spores to cause meiotic drive.
    Article Snippet: We then ran the proteins on NuPAGE 4–12% Bis-Tris protein gels (Life Technologies) in 1 x MOPS buffer (Life Technologies) for 50 min- utes at 200V and then transferred to PVDF membranes (Bio-RAD #1704156) using the TransBlot Turbo system.

    Article Title: Comparative Proteomic Analysis Reveals Varying Impact on Immune Responses in Phorbol 12-Myristate-13-Acetate-Mediated THP-1 Monocyte-to-Macrophage Differentiation
    Article Snippet: Protein samples were run on pre-cast NuPAGE Bis-Tris gels (Invitrogen) with 1 x MOPS buffer (Invitrogen) and transferred on nitrocellulose membranes, using iBlot 2 Gel Transfer Device (Invitrogen).

    Article Title: Dose-dependent phorbol 12-myristate-13-acetate-mediated monocyte-to-macrophage differentiation induces unique proteomic signatures in THP-1 cells
    Article Snippet: Protein samples were run on pre-cast NuPAGE Bis-Tris gels (Invitrogen) with 1 x MOPS buffer (Invitrogen) and transferred on nitrocellulose membranes, using the iBlot2 Gel Transfer Device (Invitrogen).

    Article Title: CDNF protects the nigrostriatal dopamine system and promotes recovery after MPTP treatment in mice
    Article Snippet: The primary antibody was visualized using the AlexaFluor 488 goat anti-mouse or AlexaFluor 568 goat anti-rabbit secondary antibody (Invitrogen).

    Article Title: Proteomic Analysis of Mecistocirrus digitatus and Haemonchus contortus Intestinal Protein Extracts and Subsequent Efficacy Testing in a Vaccine Trial
    Article Snippet: To determine the complete protein profile from each parasite, individual non-reduced Novex NuPAGE 4–12% Bis-Tris gels (Life Technologies) for M. digitatus and H. contortus in 1 X MOPS buffer (Invitrogen) were run at 200 V for 45 mins following the manufacturers' protocols.

    BIA-KA:

    Article Title: UMP-CMP kinase 2 gene expression in macrophages is dependent on the IRF3-IFNAR signaling axis
    Article Snippet: .. Protein concentrations were measured by BCA assay (Pierce, Waltham, MA), and samples were resolved on NuPAGETM Bis-Tris gels (Invitrogen, Bleiswijk, Netherlands) with 1 x MOPS buffer (Invitrogen, Bleiswijk, Netherlands) followed by transfer on nitrocellulose membranes, using the iBlot12 Gel Transfer Device (Invitrogen, California, United States). .. After washing the membranes in TBS-T (Tris Buffered Saline with 0.1% Tween-X100), blocking of membranes was carried out with 5% BSA for 1hour and then probed with the following primary antibodies overnight at 4°C under gentle agitation: anti-β-tubulin (Abcam, ab15568, Cambridgeshire, England), CMPK1 Monoclonal Antibody (OTI1H8; MA5-26124, Invitrogen), and TYKi Antibody (Novus; NBP1-80653, Oxfordshire, England) GAPDH (Abcam, ab8245, Amsterdam, Netherlands), Anti-COX IV-mitochondrial loading control (Abcam, ab16056, Amsterdam, Netherlands), Phospho-Stat1 (Tyr701) (58D6; CST-#9167, Cell Signaling Technology, Leiden, Netherlands), Phospho-TBK1/NAK (Ser172) (D52C2; CST-#5483, Cell Signaling Technology, Leiden, Netherlands), MX1 (D3W7I; CST-#37849, Cell Signaling Technology, Leiden, Netherlands), and ISG15 (CST-#2743, Cell Signaling Technology, Leiden, Netherlands).



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